The flow cytometer Diaries
The flow cytometer Diaries
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Info-enriched characterization of mobile populations. Morphological info from pictures can add to your richness of flow cytometry knowledge. For example, the figure shows an or else common apoptosis assay using Annexin V and PI, introducing mobile imaging to characterize cells in Every single inhabitants to expose morphologically distinctive capabilities. These insights could not are actually attained from multiplex staining alone.
As samples are obtained on the Attune CytPix Flow Cytometer, the higher-velocity brightfield camera captures and suppliers images of detected activities in a price as many as six,000 photos per 2nd, dependant upon the flow amount and graphic sizing. For bigger flexibility, Attune Cytometric Software program permits you to adjust the image capture frequency as wanted and select a specific gate for picture capture, if essential.
Blend acoustic focusing technologies with superior-parameter Evaluation for versatile spectral unmixing and standard workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing which has a superior-speed brightfield camera for simultaneous substantial throughput flow cytometry and superior resolution brightfield imaging.
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Furthermore, it can be illuminating to discover the amount of doublets slip via classic singlet gates, and wonderful to validate the standing of unpredicted double-beneficial functions in my Examination."
Pair your Attune flow cytometer using an autosampler for elevated workflow performance, and incorporate robotic integration for max throughput.
Our experts will structure panels to suit your needs as often times you will need—totally free, no product or service obtain necessary.
The Attune NxT cytometer will be the trustworthy workhorse instrument your lab can trust in for dependable and reliable overall performance. The Attune CytPix model features a substantial-speed brightfield camera and AI-pushed picture Examination software, with modern upgrades which includes machine Studying resources and an intuitive person interface, enabling Improved facts precision, accuracy, and a lot quicker insights into sample biology.
Probably most of all, these functions include cells of clearly diverse phenotypes which may lead to incorrect conclusions pertaining to double optimistic activities (particularly in rare populations).
Sound waves permit exact cell alignment, causing Remarkable info good quality and ~10X speedier sample flow rates.
Software note: Automated impression analysis minimizes user-to-person variability in flow cytometry gating techniques
Leverage the strength of State-of-the-art technological know-how and innovation to accelerate your discoveries. Have confidence in while in the Attune Flow Cytometer household to provide the dependability, speed, and precision that set your perform aside.
Adaptable payment approach from Agilent Money Alternatives enable you to have the significant instrumentation you may need for speedy challenge, or to improve your lab.
Gating on these extended parameters lets you immediately and correctly detect populations of curiosity and make sure gating technique with little if any manual evaluation. The Attune software program delivers entry to condition-of-the-art machine Finding out Investigation instruments inside of an expertly developed consumer interface, which makes it exceptionally easy to use.
As the mobile line had previously been analyzed around the Attune CytPix Flow Cytometer, the scientists went back again to the pictures and had been capable of document the microbial an infection not less than 5 times before.
The Attune CytPix Flow Cytometer permits consumers to visualize samples in the course of acquisition, validate the precision of gating approaches, and guidance results with trusted documentation for publication functions.
Listed here, we exhibit that by gating tactics making use of image-dependent click here quantitative parameters can distinguish interacting cells from coincident situations to additional precisely evaluate interacting cells.